ˎƷQ
ͨQȱݲw\ԇ(ø(lin)߷)
ӢQDiagnostic Kit for Antibody to Human Immunodeficiency Virus (ELISA)
hZƴRenlei Mianyi Quexian Bingdu Kangti Zhenduan Shijihe ( Meilianmianyifa)
bҎ(gu)
96˷/
A(y);
ѪҺĺYRHIVȾo\
zԭ
EIApԭAķzyA(y)HIVԭķ(yng)׃(ni)y(bio)(bio)п-HIVtװγɿԭ-w(f)cø(bio)ԭY(ji)γɿԭ-w-ø(bio)ԭ(f)(jng)@ɫϵy(tng)@ɫ(j)OD ֵжПoHIVwĴ
ҪMɳ
1HIVԭ 96ס1K
2øY(ji) 12ml1ƿ
3HIV-1Ԍա 1.0ml1ƿ
4HIV-2Ԍա 1.0ml1ƿ
5Ԍ 1.0ml1ƿ
6sϴҺǰÿƿ1:20ϡ 50ml1ƿ
7@ɫA 6ml1ƿ
8@ɫB 6ml1ƿ
9KֹҺ 6ml1ƿ
10z 2Ƭ
11f 1
lЧڡ
28ܹⱣ12
mÃx
1Һ͝^
237ˮԡ
3Ӌr
4ϴC
5ʎ
6ø(bio)x zL450nmL630nm
ӱҪ
1ӱɼҪ(zhn)IJѪg(sh)ռѪҺȫѪƷȷ37o2СrٌѪƷx3000D(zhun)/x10ʹѪѪ{OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сrϡ
2ӱʹѪѪ{ػEDTAȿӰԇY(ji)
3ӱкBcӰԇY(ji)߶ѪĘӱ]ȫտsѪӱȾĘӱܕe`ĽY(ji)
4rĘӱ28ol¿ɱһLڃ-20£ⷴ(f)
z
1ÿԇO(sh)ԃɿHIV-1ԌպHIV-2ԌոɿףքeԌ100lO(sh)һհ100lyӱ3760ȥ׃(ni)Ʒ۸
2ϴ壺
1ֹϴ壺ȥ(yng)l׃(ni)ҺwˮĸɣϴҺעMÿo510룬ȥ׃(ni)ϴҺĸ˷(f)5ĸ
2ϴCϴ壺xϴ5εijϴϴҺ(yng)עMÿף_ÿoˮĸ
3ÿøY(ji)100lհտײӣ3730ͬϷϴ(yng)5Ρ
4ÿ@ɫAҺBҺ50lppʎ37ܹo30犡
5 ÿKֹҺ50lKֹ(yng)ø(bio)xx(sh)x 450nmԿհУ㣩pL450/630nmxȡODֵx(sh)ڽKֹ(yng)10犃(ni)
ֵ
1ԇO(sh)ӋҪHIV-1ԌպHIV-2Ԍƽֵ1.20ԌƽֵС0.10ԌODֵ֮(yng)1.20tԇoЧ
2Ԍx(sh)С0.05r0.05Ӌ
3RֵCut off value=Ԍƽֵ+0.10
4yԇ(bio)ODֵСRֵtHIVw
5yԇ(bio)ODֵڻRֵtHIVwע⣺ԇԘ(bio)(yng)ȡMp(f)ԇ(f)ԇߑ(yng)ȫ̲zyҎ(gu)HIV_CMд_Cԇ
zY(ji)Ľጡ
1ԽY(ji)ӱв-HIVӱе-HIVԇеęzy
2ԽY(ji)ӱк-HIVخ(yng)ء
3ԽY(ji)ĘӱҪM؏(f)ԇֻн(jng)^؏(f)؏(f)ԇԞԽY(ji)ĘӱJ(rn)HIVԡ؏(f)Y(ji)ԵĘӱ(yng)(jng)^m(dng)?sh)ֶMд_J(rn)
ԽY(ji)Ęӱ(f)zԽY(ji)(yng)J(rn)Ԙӱ؏(f)ԽY(ji)܌һg(sh)}£
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥص
ԇľԡ
ƷHڙzyѪѪ{-HIVRȱݲȾo\
a(chn)Ʒָ(bio)
1zyHIVwԅƷ(yng)Ҫ
2zyHIVwԅƷ(yng)Ҫ
36HIVw͙zޅƷԷ(yng)3һ|(zh)Ѫ
41HIVwҾԅƷMЙzCV(%)(yng)15%(n=10)
5(wn)ԌԇM376zY(ji)(yng)_(bio)(zhn)
ע헡
1 ƷHw\HڙzywѪ塢Ѫ{zyY(ji)\ָʾ֮һ
2 28lȡĘӱ(yng)Ҝƽ30MЙzy
3 횴_ƷӘĜ(zhn)_ӘӲ(zhn)_ܕ(do)e`ԇY(ji)
4 ʹҺֹӘӕr(yng)ÿθQ^ȡӱ
5 ڲ^Б(yng)Mⷴ(yng)Юa(chn)
6 sϴҺϵߝ}ܕγɽY(ji)δȫܽ⣬ӰԇY(ji)ͬrܕסԄӼӘϴCܵF(xin)Y(ji)Ո37ܽȻϡጻʹá
7 z؏(f)ʹ
8 ˮԡ偕rՈ(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37
9ϴ壺
1ϴCϴrϴCļҺP(gun)ҪȱϴҺ^ܳM(yng)УϴΔ(sh)(yng)ԓ5(jng)עzҺ^Ƿ
2ֹϴrՈʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝ(zhn)_
3ϴrõˮՈ(f)ʹá
4ϴCÿʹǰsˮȥxˮϴɃԷֹܵg
10ʹȫԄø(bio)ϵy(tng)ܕϵy(tng)`(yng)ϵy(tng)(sh)O(sh)m(dng){(dio)ԝMP(gun)|(zh)ƷҪ
11κԇ|Ƥw۾ôˮԓλMДUϴKֹҺиg
12ԇЃ(ni)P(gun)MݼRӱҕНڂȾԣՈP(gun)ČҹҎ(gu)(zh)к̎