a(chn)ƷQ
ͨQײIgGwzy(c)ԇУø(lin)߷
bҎ(gu)
48 ˷/96 ˷/
A(y);
a(chn)Ʒwⶨԙzy(c)ѪײIgGw
(sh)(yn)Y(ji)HRR\εΨһ(j)ײȾo\
ϢB
ײHEVСs2734nmĆι期oĤRNAMȫL(zhng)s7.5kb3(g)_xaܣORFORF1ac(f)P(gun)ĹԵףORF2cORF3Ɯy(c)aĽY(ji)(gu)ORF2 C˾aҪY(ji)(gu)ҪĿԭλc(din)о߂ͨ^(du)ϳĿԭؽMԭоl(f)F(xin)˶(g)ԭλоORF2ؽMԭcϳĿԭELISAzy(c)HEVwR\
((jin)Q) ײHepatitis E Virus, HEVȾ¡RIJʸڼҪڰl(f)չЇ(gu),DŮȾHEV䲡ɸ_(d)20%,3(g)µDŮ߿_(d)56%YоһNȾDZo(j)80ĩl(f)F(xin)µIJԸ֮һȫVҊځޡc^(q)҇(gu)DzԸ߰l(f)^(q)(j)y(tng)Ӌ(j)ڼԲԸװl(f)ʞ3.420.5ƽ8.6
GENELABS®ײIgMIgG ELISAzy(c)ԇΨһ@Ç(gu)ˎƷM(jn)ע(c)Cײzy(c)M(jn)ԇ GENELABSHEV IgMIgGԇвø(lin)ײMضйԵĻƬքezy(c)wѪѪ{пHEV IgMIgG@N(bio)־ؽMƬγɹؑ(yng)ڲͬĵطЅ^(q)ļȾ\ѪW(xu)HEV IgMIgGzy(c)\ײȾҪָ(bio)֮һ
z(yn)ԭ
ƷɻؽM_(d)ײHEVԭװӴy(c)Ѫ, (dng)y(c)ƷдײwtγɿԭwخY(ji)ټ뿹IgGøY(ji)c䷴(yng)(jng)ø@ɫø(bio)xϜy(c)(j)ODֵжПoײ IgGwĴ
ҪMɳ
1ײԭװ |
96ס1K |
48ס1K |
2øY(ji) |
10mL1ƿ |
5mL1ƿ |
3Ԍ(du) |
1mL1ƿ |
1mL1ƿ |
4Ԍ(du) |
1mL1ƿ |
1mL1ƿ |
5ϴҺǰ120ϡጣ |
50mL1ƿ |
50mL1ƿ |
6@ɫ A |
6mL1ƿ |
3mL1ƿ |
7@ɫ |
6mL1ƿ |
3mL1ƿ |
8KֹҺ |
6mL1ƿ |
3mL1ƿ |
9ƷϡҺ |
10mL1ƿ |
5mL1ƿ |
(10) zƬ |
3 |
3 |
MݽMɣ1ײԭװ壺ûؽM_(d)ײ14͵ORF2ORF3λԭϰϩװ壻2øY(ji)50%СţѪ15%ɽѪ壬0.1%0.01M}_Һ(pH7.4)3Ԍ(du)գײIgGwzy(c)Ѫ4Ԍ(du)գײIgGwzy(c)Ѫ壻5ϴҺ}_Һ-0.5206@ɫ A8.00g ,^0.70g; 7@ɫB2.10gҶ0.38g3,3',5,5'-ļ(lin) 0.40g8KֹҺ2M H2SO49ƷϡҺ0.24%u飨Tris_Һ1.78%NaCl0.5%ҵc0.2%ţѪ0.1%-200.1%ͨX-1005%ɽѪ0.1%
(ch)lЧ
ԇБ(yng)2~8бܹⱣЧ12(g)δİװl(yng)Էܷcʣԇ2~8汣棬һ܃(ni)\(yn)ݔ5첻Ӱ䷀(wn)
a(chn)ڼЧҊ(bio)
mÃx
ø(bio)xz(yn)L(zhng)450nmL(zhng)630nm
ӱҪ
1ӱɼҪ(zhn)IJѪg(sh)ռѪҺȫѪƷȷ37̎2Сr(sh)ٌѪƷxģ3000D(zhun)/x6ʹѪ岻OٺѪ(x)鿹ѪƷ(yng)ѪƷxĺٷҜ2Сr(sh)
2ӱʹѪ壬ػEDTAȿ(hu)Ӱ푌(sh)(yn)Y(ji)
3ӱкïBc(hu)Ӱ푌(sh)(yn)Y(ji)߶ѪĘӱ]ȫտsѪӱȾĘӱܕ(hu)e(cu)`ĽY(ji)
4rĘӱ2~8±һrӱL(zhng)ڃ(ch)-20£ⷴ(f)
z(yn)
1(sh)(yn)(zhn)䣺حh(hun)ȡԇҜƽ30ͬr(sh)sϴҺ1:20ϡጡ
2Ӵy(c)ӱÿΌ(sh)(yn)O(sh)Ԍ(du)աԌ(du)ոɿքeꎡԌ(du)Ѫ100LO(sh)һհ(du)ӘƷϡҺ100L100LƷϡҺټӴy(c)Ѫ10Lֻ3730ȥ׃(ni)ӱ۸
3ϴ壺1ֹϴ壺ȥװl׃(ni)ҺwˮĸɣϴҺעMÿo5~10ȥ׃(ni)ϴҺĸ˷(f)5ĸɡ
2ϴC(j)ϴ壺xϴ5εijϴϴҺ(yng)עMÿ_ÿoˮĸ
4øY(ji)ÿ100Lհ(du)տײֻNճ3720犡
5ϴ壺ͬ3
6@ɫȼ@ɫAҺ50L ټ@ɫBҺ50Lֻ37 ܹ10
7Kֹ(yng)ÿӽKֹҺ50L
8y(c)ø(bio)xx(sh)xÆβL(zhng)450nm(ԿհУ)450/630nmxȡODֵx(sh)ڽKֹ10犃(ni)
Дֵ
Cutoff ֵӋ(j)㣺COV=Ԍ(du)ƽODֵ0.12
(bio)ODֵCOV(bio)ODֵ
עԌ(du)ODֵ0.050.05Ӌ(j)0.05(sh)HODֵӋ(j)
z(yn)Y(ji)Ľ
1ԽY(ji)ӱвײIgGwӱеײIgGwԇеęzy(c)
2ԽY(ji)ӱкײIgGwخ(yng)ء
3ԽY(ji)ĘӱҪM(jn)؏(f)(sh)(yn)ֻн(jng)^؏(f)؏(f)(sh)(yn)ԞԽY(ji)ĘӱJ(rn)ײIgGw؏(f)Y(ji)ԵĘӱ(yng)(jng)^m(dng)?sh)ֶM(jn)д_J(rn)
ԽY(ji)Ęӱ(f)zԽY(ji)(yng)J(rn)Ԙӱ؏(f)F(xin)ԽY(ji)܌һ(g)(g)g(sh)}£
1 ڃxӘɵĽȾ
2 @ɫxȾ
3 ԇ©ɵĽȾ
4 ֵϴȥص
4ԇڼԡl(f)Ŀ93.8%خ94.4%
ԇ(yn)ľ
ƷHڙzy(c)ѪײIgGwwzy(c)Ro\
a(chn)Ʒָ(bio)
1.ԇ^Һwo
2.10ײwIgGԅƷzy(c)(yng)ȫz
3.10ײwIgGԅƷzy(c)(yng)ȫz
4.4ײwIgG`ȅƷzy(c)S1S2S3 (yng)S4
5.1ײwIgGԅƷzy(c)CV%(yng)15n=10
6.(wn)Ԍ(sh)(yn)ԇM376zY(ji)(yng)_(d)(bio)(zhn)
7.bݘ(bio)ʾb
8.֬ѪSȸɔ_ӌ(du)zy(c)Y(ji)Ӱ푣Ȟ2.1mmol/L(ֵ0.22~1.65mmol/L)ѪtםȞ210g/L(ֵ110~170g/L), đ̴Ȟ8.5 mmol/L(ֵ:2.6~6.5mmol/L)Ӱ푱Ʒzy(c)Y(ji)
9.cԭwĽ淴(yng)о:HAV16HBV23HCV24HIV26TORCH 18M(jn)н淴(yng)ԇ(yn)Y(ji)Ӱ푱Ʒęzy(c)Y(ji)
10.R(du)оϢ(jin)飺оԇc(gu)(zhn)ײIgGwzy(c)ԇ(du)R(bio)M(jn)Ќ(du)șzy(c)^خԵȷĵЧ
(xing)ĿӱĴ_(j)(j)w\ԇע(c)kҎ(gu)оa(chn)Ʒa(chn)Ʒ(j)w\ԇRоg(sh)ָ(do)ԭtRоӱ(sh)1314(du)оY(ji)93.8%;خ94.4%;ӱ94.1%
ע(xing)
1 ƷHw\Hڙzy(c)wѪ壬zy(c)Y(ji)HRR\εΨһ(j)
2 2~8lȡĘӱ(yng)Ҝƽ30M(jn)Йzy(c)
3 횴_ƷӘĜ(zhn)_ӘӲ(zhn)_ܕ(hu)(do)e(cu)`Č(sh)(yn)Y(ji)
4 ʹҺֹӘӕr(sh)ÿΑ(yng)ԓQ^ȡӱ
5 õƿμӕr(sh)Ո(qng)ƿҺwuȥ1~2κֱٵصμҪ⌢MеҺw|?yn)Rװ߅ϣeøY(ji)
6 ڲ^Б(yng)Mⷴ(yng)Юa(chn)
7 sϴҺϵߝ}ܕ(hu)γɽY(ji)δȫܽ(hu)Ӱ푌(sh)(yn)Y(ji)ͬr(sh)ܕ(hu)סԄ(dng)ӘϴC(j)ܵF(xin)Y(ji)Ո(qng)37ܽȻϡጻʹ
8 z؏(f)ʹ
9 ˮԡ偷(yng)r(sh)Ո(qng)(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37档
10ϴ壺1ϴC(j)ϴr(sh)ϴC(j)ļҺP(gun)ҪȱϴҺ^ܳM(yng)УϴΔ(sh)(yng)ԓ5(jng)עzҺ^Ƿ
2ֹϴr(sh)Ո(qng)ʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zy(c)Y(ji)Ĝ(zhn)_
3ϴr(sh)õˮՈ(qng)(f)ʹ
4ϴC(j)ÿʹǰsˮȥxˮϴɃԷֹܵg
11ʹȫԄ(dng)ø(bio)ϵy(tng)ܕ(hu)ϵy(tng)`(yng)(du)ϵy(tng)(sh)O(sh)m(dng){(dio)ԝM(gu)P(gun)|(zh)ƷҪ
12κԇ|Ƥw۾ôˮ(du)ԓλM(jn)ДU(ku)ϴKֹҺиgԡ
13ԇЃ(ni)P(gun)MݼRӱҕНڂȾՈ(qng)P(gun)Č(sh)(yn)ҹҎ(gu)(zh)к̎
īI(xin)
1.R(sh)(yn)҅Ķxʹ_
2. 2006|ϴW(xu)ġȫ(gu)Rz(yn)Ҏ(gu)̡
3.RW(xu)w\ԇУͨüg(sh)Ҫ
t(y)еa(chn)I(y)SC̖(ho)
t(y)еע(c)C̖(ho)/a(chn)Ʒg(sh)Ҫ̖(ho)
f˜(zhn)ڼڡ