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͸ײĿԭzyԇУø“(lin)߷_ϺsʢˎI(y)ɷ޹˾
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͸ײĿԭzyԇУø“(lin)߷

͸ײĿԭzyԇУø“(lin)߷

a(chn)ƷQ

ͨQ:͸ײĿԭzyԇУø“(lin)߷


bҎ(gu)

96˷/


A(y);

a(chn)ƷwⶨԙzyѪѪ{еı͸ײxĿԭ

pwAø“(lin)߷ԭELISAԙzyѪѪ{Ʒеı͸ײxĿԭozyá

͸ײHepatitis C VirusHCVݔѪ󲡶ԷǼ׷͸׵ҪԭҪͨ^ݔѪo}Ҳͨ^н|ĸĿǰȫs1.31.7|˞ԱβȾÿ35fc͸P(gun)ĸK(j)Ӌ(j)ÿİf˸Ⱦβ

βȾЕr(sh)(do)¼԰Yć(yn)س̶ȿȾβпֻF(xin)p΢YҲܽKЇ(yn)Ըβ܌(do)¸Ӳ͸ΰȾs80%˲F(xin)κΰYܳF(xin)ļ԰Yl(f)ȫʳ½ġIʹɫɫ׃\P(gun)(ji)ʹS㣨Ƥw۰װl(f)SԸȾ߳F(xin)Yܱ仼ڸβһ15~20%ҵHCVȾߞԸȾ^80~85%ĸȾ߰l(f)չԱ͸20%Ŀɰl(f)չwSK4~5%ĸwS߰l(f)μ(x)ԸΰΣʮև(yn)ء͸ɱβĸβ

͸׺Ŀԭzy½l(f)չһNHCVȾęzyڸȾHCVHCVĿԭF(xin)^cѪHCV RNAdP(gun)HCVȾ򿵏(f)ʧHCVȾ\ࡢI(xin)ѪTYHCVȾ\ΣȺO(jin)y߹ϵKߵHCVȾ\beȾֻF(xin)YȾڿЧA(y)yͱO(jin)y


z(yn)ԭ

ƷɻؽM_(d)ı͸ײą^(q)ԭHCV-cAgСƂĆο¡w΢װø(bio)ӛHCV-cAgο¡wԌѪȽM(yng)pwAø“(lin)߷ԭELISAzyѪѪ{Ʒб͸ײxĿԭ


ҪMɳ

1

w

96˷1K

2

øY(ji)

20mL1ƿ

3

ԌѪ

0.5 mL1ƿ

4

ԌѪ

0.5 mL1ƿ

5

ϴҺʹÕr(sh)20ϡጣ

50mL1ƿ

6

 A

6mL2ƿ

7

 B

6mL2ƿ

8

KֹҺ

6mL1ƿ

9

ƷϡҺ

10mL1ƿ

10

3

ע⣺̖ͬĽMֲûá


MҪMɣ1w壺ᘌĿԭĆο¡w2øY(ji)u׻龏_ҺɽѪţѪ׵3ԌѪ͸ײĿԭzyѪ壻4ԌѪ͸ײĿԭzyԵѪ5 ϴҺNaClͨ X-1006Aˮc^7BᣬҶļ׻“(lin)8KֹҺ9ƷϡҺ Tris _ҺNaClҵcţѪ׵ףœ-20ͨX-100ɽѪ  


Ʒ̓x

1rƂsˮȥxˮ

2һף

3_΢Һ͝^

4Ƀˮ

537ˮԡ

6Ӌ(j)r(sh)

7ϴC(j)ϴƿ

8ʎ

9ø(bio)x


28ܹⱣЧ12(g)

a(chn)ڼЧҊ(bio)


mÃx

Ԅø(bio)yxpLr(sh)O(sh)L450nmL630nmβLr(sh)O(sh)ÙzyL450nmO(sh)ÿհ׌{(dio)


ӱҪ

    1ӱʹѪѪ{cػEDTAȿӰ푌(sh)(yn)Y(ji)

2ӱoƂ̎_t(y)üg(sh)ɼȫѪӱoðСr(sh)Ϻ3000D(zhun)/x10ַxѪʹѪ岻Oٺt׼(x)tܕ(do)¼ԽY(ji)

3ʹüӟ̎Ęӱ֬Ѫӱ(yng)(dng)ڌ(sh)(yn)ǰóxķӱ

4S(yn)ȾĘӱ(do)e(cu)`ĽY(ji)

5ӱкЯBcӰ푌(sh)(yn)Y(ji)ïBcӱ

6ѪӱδǰxČ(do)wS׵ĴwS׌Y(ji)Ӱ횴_x̎ǰӱѽ(jng)ڽܿίIJ˘ӱҪLr(sh)g


z(yn) 

1(sh)(yn)(zhn)

1حh(hun)ȡԇҜƽ30

2ϴҺƣԇеϴҺsϴҺںmĝ(ni)rƂsˮȥxˮsϴҺϡ20

sϴҺߝ}_Һڜضȵ͕r(sh)ܕнY(ji)Ո?jin)ʹǰ_sϴҺoY(ji)l(f)F(xin)нY(ji)ՈˮԡмӟܽtӰϴЧ

2

1ÿΌ(sh)(yn)ڰO(sh)һ(g)հ׿O(sh)Ԍոp

2ԌѪO(sh)ɿמԌգÿ׼ԌѪ200L

3ԌѪO(sh)ɿמԌգÿ׼ԌѪ200L

4ӘƷϡҺÿףԌտףм100LƷϡҺհ׿׼200LƷϡҺ

5ӘӣƷÿ׼ƷѪѪ{100L

6òzƬ371ԡʎ60

7ϴ죺СĽҵĤȥ(yng)l׃(ni)ҺwϴҺҺעMÿÿב(yng)벻400LϴҺҺo510ȥ׃(ni)ϴҺ؏(f)5ΡڸɃˮĸ

8øY(ji)ÿ׼200LøY(ji)

9òzƬ371ԡ30

10ϴ죺ͬ7

11@ɫÿȼ100LAټ100LB371ԡܹ@ɫ15

12Kֹ(yng)ÿ׼50LKֹҺֻ

13 Y(ji)yY(ji)ڷ(yng)Kֹ10犃(ni)M(jn)Мyʹø(bio)xyֵxβL450nmO(sh)հ׿{(dio)㣩xpLL450nmL630nmxȡODֵ


Дֵ

CUT OFF_Ԍƽֵ+0.06ԌODֵ0.06r(sh)0.06Ӌ(j)㣩

ԌÿODֵ>0.8ԌODֵÿף0.1r(sh)yY(ji)Ч

Y(ji)ж

1ODֵCUT OFFжHCVĿԭ

2ODֵRֵCUT OFFжHCVĿԭ

zԵĘƷ(yng)M(jn)p׏(f)zԴ_J(rn)挍(sh)Y(ji)


z(yn)Y(ji)Ľ

1ѪӱδȫѪ{ӱwS׻ӱ(yn)ȾY(ji)Ӱ

2(sh)(yn)ʹLr(sh)gA򷴏(f)ڵĘӱԇęzy`Ӱ푣hʹrӱM(jn)Ќ(sh)(yn)


z(yn)ľ

1ԇЃHڶԙzyѪѪ{ƷеHCVxĿԭ

2ELISA(yng)ԭƣԇzyY(ji)ԲȫųHCVȾĿܣzyY(ji)Ա횽Y(ji)ϴ_Cԇָ(bio)RϢM(jn)з


a(chn)Ʒָ(bio)

15I(y)HCVĿԭԅѪzyY(ji)óF(xin)ԣ4݇HCVĿԭԅѪzyԅƷʣ+/+(yng)3/4

10I(y)HCVĿԭԅѪzyY(ji)óF(xin)4I(y)͙zޅƷzyS1S2 S3(yng)S45͙݇zޅƷzyL1L2 L3(yng)L4pL5Ի   

I(y)ҾԅƷ؏(f)10Ιzyп׌(sh)(yn)Y(ji)CV%15%n=10

(wn)ԇ(yn)M376(xing)ָ(bio)(yng)Ҏ(gu)

2ԇzy͸ײĿԭ`Ȟ1pg/mL

3淴(yng)(sh)(yn)HBVHIVHAVԘӱӰ푱ԇЌHCVĿԭęzy25RFߝԘӱ23ANAߝԘӱքeñԇM(jn)HCVĿԭzyӱԔ(sh)1خԷքe96.0%95.7%

4Rо60ͬԴѪͬһߵѪѪ{zyY(ji)ȫһ


ע(xing)

128lȡĘӱ(yng)Ҝƽ30M(jn)Йzy

2횴_ӘĜ(zhn)_ӘӲ(zhn)_ܕ(do)e(cu)`Č(sh)(yn)Y(ji)

3ʹ΢ҺֹӘӕr(sh)ÿΑ(yng)ԓQ^ȡӱ

4ڲ^Б(yng)M΢Юa(chn)

5sϴҺϵߝ}ܕγɽY(ji)δȫܽӰ푌(sh)(yn)Y(ji)ͬr(sh)ܕסԄӼӘϴC(j)ܵF(xin)Y(ji)Ո37ܽȻϡጾʹ

6z؏(f)ʹ

7ˮԡ偷(yng)r(sh)Ո(yng)ˮ1/3ײԾW(wng)֧֧ˮؿ37

8ϴ壺1ϴC(j)ϴr(sh)ϴC(j)ļҺP(gun)ҪϴҺ^ܳM(yng)΢УϴΔ(sh)(yng)5(jng)עzҺ^Ƿ

2ֹϴr(sh)ՈʹÎмˮİԷԴ^ø߀ԭ|(zh)c@ɫl(f)(yng)Ӱ푙zyY(ji)Ĝ(zhn)_

3ϴr(sh)õˮՈ𷴏(f)ʹ

4ϴC(j)ÿʹǰsˮȥxˮ_ϴɃԷֹ·g

9κԇ|Ƥw۾ôˮԓλM(jn)ДU(ku)ϴKֹҺиg

10ԇЃ(ni)P(gun)MݼRӱ(yng)ҕНڂȾԣՈP(gun)Č(sh)(yn)ҹҎ(gu)(zh)к̎